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← All articlesEditorial brief · abstract-levelScore 56/100Confidence medium
biorxiv2026-08-11mtDNAevolutionpathogen genomics

Colletotrichum cereale’s first chromosome-scale genome includes a mitochondrion and three huge accessory chromosomes

A 58.01 Mb Nanopore/Hi-C/Illumina assembly of C. cereale isolate 6B yields 13 chromosome-scale scaffolds plus a mitochondrial genome (N50 5.44 Mb, 98.6% BUSCO). Three AT-rich, gene-poor accessory chromosomes (2.71, 1.86, 1.36 Mb) are the first chromosome-scale accessory set in this grass anthracnose fungus and among the largest in the genus. They carry predicted effectors, CAZymes, and BGCs and show wild presence-absence variation across isolates. Core genomes still split clades A and B (ANI ~92–99.9%). Mitochondria are an assembled circle in a pathogen genome paper.

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Signal profile (abstract-level)

mtDNA · evolution · pathogen genomics

Score 56/100BIORXIVmedium confidencemtDNA
56
Importance
50
Mito signal
25
Dysfunction
75
Evidence
15
Translational

Editorial signal profile from the abstract (importance score, mito keywords, dysfunction tags, evidence density, translational cues). Not a figure reproduced from the preprint PDF.

Finding. The grass anthracnose fungus Colletotrichum cereale now has a finished 58 Mb, 13-chromosome genome and a mitochondrial genome to go with it. Three of those chromosomes are accessory, AT-rich, and large (up to 2.71 Mb), among the biggest extras in the genus, stuffed with predicted effectors, CAZymes, and biosynthetic clusters. Other isolates do not share the same extra set. The core still splits the known A and B clades, almost to species-level ANI in places. Mitochondria are present as an assembled organelle genome, not as a story.

Why this paper matters

Fragmented drafts hid chromosome architecture. Accessories this large, this variable, explain how one named species can be a moving target in turf and cereals. A mitochondrial desk files the mtDNA mention and leaves the pathogenologists the rest.

What they actually measured

Chromosome-scale assembly, accessory definition, PAV, clade ANI.

How to read the score

  1. Confidence is medium-high for the assembly claims.

What to do with it

If you study Colletotrichum or accessory chromosomes, take 6B as the reference. Pull the mitogenome only if you need it; they did not analyze it here. The directional implication is that C. cereale’s pathogenicity toolkit sits on a dynamic, discontinuously shared accessory genome.

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Source preprint

A chromosome-scale genome of Colletotrichum cereale reveals a large, dynamic accessory genome within a deeply structured species

10.64898/2026.08.06.743313

Cooper J, Carbone MA, Crouch JA, Cubeta MA, White JB, Shah R, Carbone I.

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