Finding. Anopheles stephensi, the urban malaria mosquito, now has an embryo-derived cell line that keeps growing, answers to a COI barcode, and carries a Y in at least some nuclei. TransIT-PRO gets DNA in. Anopheles gambiae’s polyubiquitin promoter drives best among the reporters tested. Mitochondria were used as an ID card.
Why this paper matters
Functional genomics in this vector has been tool-poor. A validated line is infrastructure. The mitochondrial content is a barcode sentence.
What they actually measured
Line establishment, COI, karyotype, transfection, promoter assay.
How to read the score
55.
What to do with it
If you do A. stephensi molecular work, try SDA-500 and the Ag polyubiquitin promoter. Ignore for OXPHOS. The directional implication is that this species finally has a genetically stamped in-vitro chassis.