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membrane potential mitochondria briefs

2 importance briefs tagged membrane potential, including the current week and the archive.

• biorxiv2026-10-09score 88

  Sub-toxic iron sulfate depolarizes neuroblastoma mitochondria, raises proton leak, and cuts hydrogen peroxide

  Mendonça, Rossignol, Amoedo, Oliveira and colleagues loaded SH-SY5Y neuroblastoma cells with 100 µM iron sulfate for 24 hours, a pulse that was not cytotoxic. Intracellular iron rose about four-fold. Mitochondria fragmented and lost membrane potential, yet mitochondrial content and OXPHOS coupling efficiency held. The only respiratory state that increased was proton leak. Total cellular oxidants and hydrogen peroxide output fell, ferritin rose, and the cells proliferated more while migrating less. The redox story is a leak-dependent drop in driving force that biophysically limits oxidant production, not a Fenton burst. The metal is loaded iron, stored in ferritin. Cluster nuclearity was not measured.

• biorxiv2026-07-28score 76

  Corilagin saves glutathione, membrane potential, and the AMPK-TFAM axis in high-glucose neuroblastoma cells

  In SH-SY5Y cells, 50 mM glucose for 24 hours depletes reduced glutathione, raises intracellular and mitochondrial reactive oxygen species, drops membrane potential, fragments the network, and increases cell death. Corilagin pretreatment opposes each of those changes and restores AMPK phosphorylation plus SIRT1, PGC-1α, and TFAM. The named redox species is reduced glutathione, one side of the GSH/GSSG couple. No metal site is identified. The abstract's wider disease framing (diabetic nephropathy, cerebral ischemia) is not the experiment it describes.

Also: redox biology · computational · metabolism · therapeutics · structural biology · immunology · aging · neurobiology